Question

Which step can occur even more rapidly by PCR?a. target DNA removed from cells and isolated b. cloning host treated with calcium chloride and receives plasmid c. target DNA and plasmid treated with the same restriction endonuclease d. desired protein is produced by cloning host e. gene is amplified by multiplication of cloning host

Answer 1

Answer:

E. Gene is amplified by multiplication of cloning host

Explanation:

PCR is a very powerful technique that can help us to amplify exponentially one gene from a very small sample of DNA.

As seen in the attached image, the process begins with a single DNA molecule, for the first cycle, that DNA molecule denaturalizes into two strands, the primers bind to their complementary sides and then the DNA polymerase builds the new DNA strands. The number of copies of the gene that can be obtained with each cycle are 2ⁿ copies, where n is the cycle number.