Question

A segment of DNA is 400 base pairs long and contains 2 restriction sites for the restriction enzyme HindIII. This DNA is cut with HindIII and then run on a gel. How many bands would you expect to see after completing DNA gel electrophoresis and staining the gel?

Answer 1

Answer:

I would expect to see 3 bands after completing DNA gel electriphoresis and staining the gel.

Explanation:

The restriction enzyme Hind3 has two Recognization sites in the 400 base pair long DNA molecule.

     As a result the restriction enzyme Hind3 with generate 2 cuts in the target 400 base pair long DNA molecule.

     The number of 2 cuts in the target DNA molecule will generate 3 DNA fragments.From this point of view it can be stated that 3 bands will form after completing DNA electrophoresis and staining the gel.

Answer 2

The amount of bands expected after completing DNA gel electrophoresis and staining the gel is a number around 133 base pairs on three separate strands.

The restriction enzyme

They recognize and bind to specific DNA sequences called restriction sites. Each restriction enzyme recognizes only one or a few restriction sites. When it finds its target sequences, the restriction enzyme will cut the double helix of the DNA molecule. Cutting is usually at or near the restriction site and occurs in a tidy, predictable pattern.

With this information, we can conclude that the amount of bands expected after completing DNA gel electrophoresis and staining the gel is a number around 133 base pairs on three separate strands.

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