Question

Which of the following best describes the fate of the cloned DNA used in a transformation experiment?
a. The DNA is integrated into a chromosome and maintained indefinitely.
b. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) indefinitely.
c. The DNA remains on a plasmid and is maintained as an episome (non-chromosomal DNA) for a day or two and is then lost.
d. The DNA is integrated into a chromosome but is degraded and lost after a day or two.

Answer 1

Answer:

The correct answer is a DNA is integrated into a chromosome and maintained indefinitely.

Explanation:

During DNA Cloning the target DNA is cleaved by restriction endonuclease result in the generation of target DNA fragments.

         A suitable vector is chosen and the DNA of that vector is cut with same restriction enzyme which lead to generate a gap in the vector DNA.

        A target DNA fragment is then integrated with the gap region of the vector and is maintained indefinitely .