Question

ompare your TLC analysis with the results of the column chromatography. More polar molecules move more slowly through silica than less polar molecules. Describe why silica gel chromatography is a good method of separating methyl orange from biphenyl. Based on the structure of Biphenyl and Methyl Orange, predict which compound will have a higher Rvalue on TLC and explain your choice.

Answer 1

Biphenyl will have a higher R value than the Methyl Orange.

Explanation:

Biphenyl is a aromatic hydrocarbon and it is a nonpolar molecule.

Methyl Orange is a organic compound with a -SO₃⁻Na⁺ polar functional group which will induce a high polarity in the compound.

You may find the chemical structures of both molecules in the attached picture.

Column chromatography, which use as stationary phase silica gel, is a good technique for separation of the Methyl Orange from Biphenyl.

Being a non-polar molecule,  Biphenyl will have a higher R value than the Methyl Orange.

To separate them you use a appropriate solvent as eluent, as exemple chloroform, and Biphenyl will elute first from the column and after that, as a separate phase, Methyl Orange will elute thus separating them.

Learn more about:

chromatography

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