Photosynthesis depends on the functioning of light-dependent and independent reactions. Light-dependent reactions begin with light absorption by PSII. If PSII stops working ATP, NADPH, and carbohydrates decrease.
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⇒ Light-dependent reactions occur in thylakoids.
The process goes as follows,
1. Luminous energy is trapped by chlorophyll in Photosystem II.
2. When the pigment molecules absorb light, electrons provided by water molecules get in a higher energy level.
3. The excited electrons go through the electron transport chain from Photosystem II to a less energetic level in photosystem I.
4. When the electrons leave photosystem II, they are replaced by new electrons extracted from the water molecules.
5. Luminous energy absorbed move the electrons from the photosystem I to another electron acceptor, from where they get transported again and used to produce NADPH molecules.
6. When electrons leave Photosystem I, they are replaced by new electrons coming from photosystem II.
7. When the water molecule breaks down, hydrogen ions remain in the thylakoid lumen, from where they are pumped to the stroma by the ATP synthase.
8. The released energy is used to produce ATP molecules.
9. Hydrogen ions go back from the stroma to the thylakoid compartment.
The final products are oxygen, ATP, and NADPH.
⇒ Light-independent reactions occur in the stroma.
The coenzyme NADPH plays a significant role during carbohydrates synthesis.
As a highly energetic molecule, NADPH helps to impulse the Calvin cycle to assimilate CO₂, and produce carbohydrates.
If photosystem II stops working, it will impede the whole photosynthetic process to happen.
PSII will not capture light, which will not excite electrons, and they will not go along the chain.
ATP, NADPH, and consequently, carbohydrates will decrease.
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