A.
Eukaryotes only have one circular chromosome that unwinds at multiple locations.
B.
Prokaryotes only have one origin of replication to initiate replication.
C.
Prokaryotes can only replicate their single circular chromosome in the nucleus.
D.
Eukaryotes can only replicate one segment of a chromosome at a time.
B is the correct answer because prokaryotes have only one origin of replication called initiation point which is the point where the circular chromosome touches the cell membrane