Question

What is the final concentration of 25 ug / UL if it is diluted in 1: 2 and then in 1:10 in terms of ug / UL and ng / UL? u=micro

Answer 1

Answer:

The final concentration in terms μg / μL is  $1.25 \mu g/\mu L$.

The final concentration in terms μg / nL is  $1250 ng\mu L$.

Explanation:

Initial concentration = 25 μg / μL

diluted concentration of 25 μg / μL by 1:2 = x

Ratio of final concentration to initial concentration = 1:2

$\frac{x}{25 \mu g/\mu L}=\frac{1}{2}$

$x=\frac{25 \mu g/\mu L}{2}=12.5\mu g/\mu L$

Final concentration after dilution of x by 1:10 = y

$\frac{y}{x}=\frac{1}{10}$

$y=\frac{12.5\mu g/\mu L}{10}=1.25 \mu g/\mu L$

1 μg = 1000 ng

$y=1.25 \mu g/\mu L=1.25 \times 1000 ng/\mu L=1250 ng/\mu L$