After nervous stimulation stops, what prevents ACh in the synaptic cleft from continuing to stimulate contraction?
A. acetylcholinesterase destroying the ACh
B. the tropomyosin blocking the myosin once full contraction is achieved
C. calcium ions returning to the terminal cisternae
D. the action potential stops going down the overloaded T tubules

After ceasing the nervous stimulation, the ACh in the synaptic cleft gets destroyed by the acetylcholinesterase, which further prevents the process of contraction.

• A neurotransmitter released by the motor neurons, which combines with the receptors in the motor endplate is known acetylcholine or ACh.

• ACh is a neurotransmitter found in various animals and humans, which is an ester of choline and acetic acid.

• The binding of the ACh with the receptors in the motor endplate results in action potential, which further instigates the sarcoplasmic reticulum to discharge the ions of calcium and stimulate troponin and thus stimulate contraction of the muscle.

• When the nervous stimulation stops, the enzyme acetylcholinesterase breaks down acetylcholine into acetyl and choline.

• Acetylcholinesterase is located in the synaptic cleft, which breaks down acetylcholine so that it does not remain attached to the receptors, as it would eventually result in unwanted contraction of the muscle.

Thus, the correct answer is option A, that is, acetylcholinesterase destroying the ACh.

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