Question

Indicate how pH and temperature affect the activity of an enzyme. (Choose all that apply)

A. Altering the shape of the substrate binding site
B. Causing a conformational change in the enzyme
C. Altering the substrate concentration
D. Altering the enzyme concentration

Answer 1

Answer:

B

Explanation:

Great variations in temperature and pH from the optimum that an enzyme needs can affect its function. pH affects ionization of the functional groups of amino acids. The interaction of charges of these amino groups helps in stabilizing loops such as those of beta sheets and alpha helices and give the protein its an appropriate shape to function.

Temperatures, on the other hand, while it does not change the ionization of the functional side groups of amino acids, it breaks these bonds of the interactions by giving the molecules enough energy to break free from these bonds hence denaturing the protein.