Question

Based on the results you observed for the iodine test and Benedict’s test, is it better to detect enzyme activity by measuring the reduction in the amount of substrate (iodine test) or by measuring the increase in the amount of product (Benedict’s test)? EXPLAIN your answer!

Answer 1

Answer:

Is better use the Benedict's test by the increase in the amount of the products if the enzyme is a reductase

Explanation:

The Benedict's test works by the reaction of the reducing sugars with the ion cupric of the reactive. If the enzyme is a reductase (degrades polysaccharides into bi o monosaccharides), it should cut the polysaccharide bond and the products would react with the Benedict's cupric ion

I hope you undestand me

Answer 2

The enzyme activity can be measured through measuring the reduction in the amount of substrate as seen in the iodine test.

Enzyme activity

Enzyme is a substance that can increase or decrease the rate of a chemical reaction with out being altered in the process.

The activities of an enzyme can be measured through monitoring the rate at which the substrate in a chemical reaction is being used up.

The iodine test is a test that is used to detect the presence of starch in a solution while Benedict test is used to detect the presence of reducing sugars in a solution.

The activities of enzymes can be measured using spectrophotometry, fluorescence, and radiolabeling.

Learn more about enzymes here:

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