Question

Which of the following statements accurately represents characteristics of NMR and x‑ray crystallography? X‑ray crystallography can be used only for larger proteins or complexes. The limitations of x‑ray crystallography and NMR are the same with respect to all proteins. NMR is preferable to x‑ray crystallography because it can be used with proteins in a wider range of molecular weights. X‑ray crystallography generally gives a more detailed (higher resolution) view than does NMR.

Answer 1

Answer:

X‑ray crystallography generally gives a more detailed (higher resolution) view than does NMR.

Explanation:

X-ray crystallography and nuclear magnetic resonance (NMR) are two major techniques for the examination of protein structure. Both of them have their advantage in use, and each of them is appropriate for a certain type of examination.

X‑ray crystallography has high resolution and it is not that much  limited by the protein molecular weight. Disadvantage is that the protein that is being examined must be in the crystal structure. Another disadvantage is that this method gives the static structure of the protein (motions are not available).

NMR advantage is that the structure of the protein is in its native state (it includes dynamics and intermolecular interactions) andd it doesm't have to be crystallized. Disadvantage of NMR is it cannot be used for large molecules (64kDa is the largest molecular mass).