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Acetyl CoA is used to synthesize fatty acids, which then bind with glycerol to form triglycerides.

Fatty acid synthesis is the process that occurs in the cytoplasm of the cell from acetyl-CoA and NADPH.  The enzymes that perform the process of synthesis are called fatty acid synthases. Acetyl-CoA is derived from carbohydrates via the glycolytic pathway (through glycolysis). Glycerol is also derived from the glycolytic pathway and it combines with three fatty acids to form triglycerides.

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