The correct answer to this open question is the following.
The separatist agreement with the Virginia company Fall Apart because English King James did not accept give the separatists a charter because they had a different religion and he did not want them to practice their religious belief systems.
The Pilgrims were English people that separated from the Church of England because it did not accept to be reformed. They were persecuted in Britain and had to hide or find new places to start a new life. That is why Pilgrims decided to travel to North America. So founders of the Jamestown in Virginia started to create their religious congregations.