You are interested in studying human ß-globin and decide to produce the protein in bacterial cells. The ß-globin gene is a relatively simple gene with a single intron. You insert the entire gene, including the gene's promoter, in a plasmid and transform the recombinant plasmid into E. coli. You are disappointed to discover that your bacterial culture does not produce the correct protein. What are some possible explanations? (Select all that apply.)
a) The bacterial cell is missing RNA polymerase.
b) The bacterial cell produces the protein but it is rapidly degraded.
c) The bacterial cell cannot splice introns.
d) The bacterial cell does not recognize the eukaryote promoter.
b, c, and d
