Question

talk about G+C content, its use for protein synthesis, importance, purpose, and future reasearch possibilities of it?

Answer 1

G+C content refers to the total amount of Guanine and Cytosine nitrogenous bases present in a certain DNA fragment or the DNA of complete cell.

There are three hydrogen bonds between Guanine and Cytosine as compared to two hydrogen bonds between Adenine and Thymine.

Importance of G+C content:

• The GC content of any gene or DNA is important because the more the GC content the more stable molecule with. This stabilization is due to stacking of bases which imparts them stabilization.
• It also plays an important role in the adaptation of molecule for different temperature.
• GC content also helps in the determination of annealing temperature of a specific primer sequence during polymerase chain reaction. The more GC content is, it indicates a high melting temperature of the DNA sequence.

Measurement of GC content:

GC content of a specific DNA sequence can be calculated by using formula:

G+C/ A+T+G+C X 100

It is expressed in percentage form.

Future research possibilities:

Some attempts were made by scientists to classify the micro organisms and find evolutionary history on the basis of GC content. For example: Actinobacteria and Streptomyces coelicolor are classified as high GC content bacteria. This area has potential in future for better understanding and  classification of prokaryotes and Eukaryotes on the basis of GC content.

Hope it help!