Question

Protein x has an absorptivity of 0.4 ml·mg-1 ·cm-1 at 280 nm. What is the absorbance at 280 nm of a 2.0 mg ·ml-1 solution of protein x? (assume the light path is 1cm).

Answer 1

Absorbance measures the ability of the substance to absorb light at a specific wavelength.

Absorbance is also equal to the product of molar absorptivity, path length and molar concentration.

The mathematical expression is given as:

$A= \epsilon l c$       (1)

where, A = absorbance

$\epsilon$ =  molar absorptivity

l = path length

c  = molar concentration.

The above formula is said to Beer's Law.

Absorptivity of protein x  = $0.4 mLmg^{-1}cm^{-1}$

Path length = 1 cm

Molar concentration = $2.0 mg mL^{-1}$

Put the values in formula (1)

$Absorbance at 280 nm = 0.4 mL mg^{-1}cm^{-1}\times 1 cm \times 2.0 mg mL^{-1}$

= $0.8$

Thus, absorbance at 280 nm = $0.8$

Answer 2

The study of chemicals and bonds is called chemistry. There are different types of elements and these are metals and nonmetals.

The correct answer is 280.

What is the absorbance of wavelength?

• Absorbance measures the ability of the substance to absorb light at a specific wavelength.
• Absorbance is also equal to the product of molar absorptivity, path length, and molar concentration.

The mathematical expression is given as:

A =dc

Where,

• A = absorbance
• c =  molar absorptivity
• l = path length
• c  = molar concentration.

Place all the values in the equation and solve the question.

$A =0.4*1*2.0\\=0.8nm$

Hence, the correct answer is 0.8nm.

For more information about the absorbance, refer to the link:-

https://brainly.in/question/9531558