Use the two gene sequences to answer the rest of the questions:
121 aactt ATG gat aac aat ccg aac atc aat gaa tga att cct tat aat tgt tta agt aac c (mistake sequence)
121 aactt atg gat aac aat ccg aac atc aat gaa tgc att cct tat aat tgt tta agt aac c (correct sequence from GenBank)
1. If the nucleotides in the gene were changed as indicated in the mistake sequence, what type of mutation would this be? Explain.
2. The DNA shown is the + strand of the double-stranded DNA that makes up the crygene. What would the mRNA sequence be when the minus-strand DNA is transcribed? Give the first 33 bases. What would the amino acid sequence be when the mRNA is translated? Give the first 10 amino acids.
3. How is the protein product of this DNA different from the Bt toxin that would be made if the gene did not have a mutation? Make a prediction as to the functionality of this protein compared to Bt toxin.
