Describe step-by-step how you would prepare 500mL of a solution containing 0.1M Tris base and 20% v/v ethanol from Tris base in solid form (MW = 121.14 g/mol) and 200 proof ethanol.
a) Measure 50g of Tris base, dissolve in 500mL of 80% ethanol, dilute with water to 500mL
b) Dissolve 6.06g of Tris base, add 100mL of 40% ethanol, dilute with water to 500mL
c) Dissolve 12.11g of Tris base, add 100mL of 40% ethanol, dilute with water to 500mL
d) Dissolve 6.06g of Tris base, add 100mL of 20% ethanol, dilute with water to 500mL
