The protein she wants to purify is known to have an isoelectric point (pi) of 8.1. A sample that contains the target protein has been equilibrated in a buffer at pH 7.0. When the biochemist adds the sample to the anion-exchange column, will the protein of interest bind to the resin?
a) Yes, because the pH is below the isoelectric point
b) Yes, because the pH is above the isoelectric point
c) No, because the pH is below the isoelectric point
d) No, because the pH is above the isoelectric point
