Complete the following sorting exercise to be sure you understand how the two break repair mechanisms are similar and different. Sort each item to the appropriate bin. 1. Uses an undamaged homologous chromosome as a template 2. Better for making complex substitutions 3. Occurs after a DNA break (including breaks made by Cas9) 4. Increases in frequency after single- strand breaks 5. Often results in nonfunctional (null) mutants 6. More error-prone; likely to cause indels and frameshift mutations 7. Simply involves ligation of broken DNA fragments Option: a. NHEJ Repair only b. HDR Repair only c. Both NHEJ and HDR Repair
