Please see the graph attached showing the temperature changes of the DNA in a reaction tube during one cycle of the polymerase chain reaction (PR). Please answer the following questions according to the graph.
a) Calculate the maximum AT that the reaction tube experiences during one cycle of
PCR.
b) Explain what happens to the DNA during stage X.
c) Primers are involved in Stage Y. State what happens to these primers during Stage Y.
) You are a forensic scientist discovered a tiny spot of blood at a crime scene. This spot of blood taken contained 100 molecules of DNA.
The sample underwent PCR cycles for 40 minutes. By using this graph, please calculate how many molecules of DNA would be present after this time.
