Wild type strains of E. coli have a tRNA with a 5’ GUA 3’ anticodon that recognizes the UAC codon for tyrosine. This is represented as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which a mutation changed the anticodon in this tRNA to 5’ CUA 3’ (creating CUA-tRNATyr). Answer the following question, with "yes" or "no" about translation elongation in each strain.
Will this tRNATyr recognize a stop codon in the A site?
Wild-type strain with GUA-tRNATyr
Mutant strain with CUA-tRNATyr
2. Wild type strains of E. coli have a tRNA with a 5’ GUA 3’ anticodon that recognizes the UAC codon for tyrosine. This is represented as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which a mutation changed the anticodon in this tRNA to 5’ CUA 3’ (creating CUA-tRNATyr). Answer the following question, with "yes" or "no" about translation elongation in each strain.
Will this tRNATyr recognize a normal UAC codon in the A site?
Wild-type strain with GUA-tRNATyr
Mutant strain with CUA-tRNATyr
3. Wild type strains of E. coli have a tRNA with a 5’ GUA 3’ anticodon that recognizes the UAC codon for tyrosine. This is represented as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which a mutation changed the anticodon in this tRNA to 5’ CUA 3’ (creating CUA-tRNATyr). Answer the following question, with "yes" or "no" about translation elongation in each strain.
Will peptide bond formation occur with this tRNATyr bound in the A site?
Wild-type strain with GUA-tRNATyr
Mutant strain with CUA-tRNATyr
4. Wild type strains of E. coli have a tRNA with a 5’ GUA 3’ anticodon that recognizes the UAC codon for tyrosine. This is represented as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which a mutation changed the anticodon in this tRNA to 5’ CUA 3’ (creating CUA-tRNATyr). Answer the following question, with "yes" or "no" about translation elongation in each strain.
Will the normal, wild type protein be produced in this strain?
Wild-type strain with GUA-tRNATyr
Mutant strain with CUA-tRNATyr
5. A point mutation occurs in which a codon is changed from AGA to AGC. Which term(s) describes this mutation? Select all that apply.
a. Deletion
b. Missense
c. Nonsense
d. Silent
e. Frameshift
f. Transversion
g. Transit
