Could please answer full:
Read the attched article titled “Use of Degenerate Oligonucleotides for Amplification of the nifH
Gene from the Marine Cyanobacterium Trichodesmium thiebautii” by:
JONATHAN P. ZEHR AND LARRY A. McREYNOLDS;
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 1989, p. 2522-2526.
Suggest a research proposal to clone the full nifH gene and confirm its sequence using the available DNA analysis tools in the gene bank.
this the style:
Title: 1 Marks
Abstract (one paragraph) 3 Marks
This is a brief description of the hypothesis and the goals of the experiment. It should indicate what questions you, as aresearcher, will be seeking to answer. An abstract provides a summary that allows readers to quickly assess the basic premise of your proposal.
Brief Introduction- 3 Marks
Material and Methods- 8 Marks
Describe your proposed experiment in depth. What processes are you going to use? What kind of equipment and supplies
will be necessary for the project? What will you use for a control, and what will be your replicate? Be thorough, but not
excessive. It might be useful to construct an outline before completing this section, as this will give you an idea of what
should be occurring when, and if your goals are attainable in the given time
Conclusion and Justification- 3.5 Marks
Your literature review will have already helped to lead the reader to an understanding of why your topic is of importance.
This is where you will explicitly state how your proposed research will advance knowledge. What are the far-reaching
effects? Will your study potentially change practices or policies? Why is it that your research deserves funding?
References- 1.5 Marks
