T7 promoter primer #69348-3 Bg/11 T7 promoter lac operator Xbal rbs AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGA Ndel Nhel T7•Tag PET-110 Ватні Bpu11021 TATACATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC TGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAA MetAlaSer Met ThrGlyGlyGin Gin MetGlyArgGlySerGlyCysEnd T7 terminator primer #69337-3 PET-116 GGTCGGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAA PET-110 GlyArgAspProAI GAI GASnLysAl ArgLysi ulaGluLeuAl Al Al a The AlaGluginEnd Ncol TACCATGGCTAGC. PET-110, GGTCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTACCATAA MetAlaser .GlyArgileArgleuleuThrLysProGluArgLysLeuSerTrpleuleuProProLeuser AsnAsnEnd T7 terminator CCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG Figure 1. Vector map of PET-11a to d cloning sites. The sequence in Figure 2 below was cut with Xba 1 and cloned into the Xbal site of PET-11a (Figure 1), if glucose was low and IPTG was added to induce gene expression how many amino acids would the resulting protein be? Xba I Xba 1 TCTAGAATGTATGGAGAGAATATTGAAAGCTAATCTAGA M Y G E NI E s Figure 2. The sequence of the MYGENIES gene. . Give the number of amino acids only in your answer (no units)
