Predict how each mutation would affect the rate of glycolysis in the liver. Mutation Glycolysis rate loss of the allosteric site for ATP in phosphofructokinase loss of the binding site for citrate in phosphofructokinase loss of the phosphatase domain of the bifunctional enzyme that controls the level of fructose 2,6-bisphosphate loss of the binding site for fructose 1,6-bisphosphate in pyruvate kinase